Table 1.
Fluorescent mutants arising during expansion of cell populations of clone 4-19 cells sorted on day 0 for nonfluorescence
| Culture vessel | Mutant frequency, n × 106
|
||||||
|---|---|---|---|---|---|---|---|
| Day 0 | Day 1 | Day 2 | Day 3 | Day 4 | Day 6 | Σ* | |
| A | 3 | 111 | 155 | 210 | 240 | 310 | |
| B | 3 | 115 | 160 | 215 | 249 | 297 | |
| C | 3 | 92 | 128 | 201 | 243 | 308 | |
| D | 6 | 79 | 156 | 210 | 246 | — | |
| E | 6 | 76 | 119 | 173 | 233 | — | |
| Mean | 4.2 | 94.6 | 143.6 | 201.8 | 242.2 | 305.0 | 991.4 |
| SEM | 0.7 | 8.0 | 8.4 | 7.5 | 2.7 | 4.1 | |
| Var | 2.7 | 320.3 | 350.3 | 284.7 | 37.7 | 49.0 | 1044.7 |
*
This column tests the expectation that the mean is numerically equal to the variance (Var) if the data have been sampled from a Poisson distribution. The closeness of the sum of the means to the sum of the variances suggests that there is no systematic deviation from the Poisson expectation.