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The 10.4 anti-PIR monoclonal antibody recognizes an allelic determinant. Bone marrow granulocytes (GR-1+/Mac-1+) from BALB/c or C57BL/6 mice were analyzed by flow cytometry staining with either the 6C1 or 10.4 anti-PIR mAbs (shaded histogram) or an isotype matched antibody (open histogram). The 6C1 anti-PIR antibody reacted with cells of both strains (Left) whereas the 10.4 anti-PIR antibody only reacted with BALB/c cells (Right).
