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Immunofluorescence analysis of the inheritance pattern of the 10.4 PIR allotope expression. Bone marrow cells from C57BL/6, BALB/c, [C57BL/6 (male) × BALB/c (female)] F1, and [BALB/c (male) × C57BL/6 (female)] F1 mice were stained with a combination of either the 6C1 (Left) or the 10.4 (Middle) anti-PIR antibodies with the anti-B220 antibody. Splenocytes were stained with anti-IgMa and anti-IgMb antibodies (Right) to verify the genetic background of the mice.
