Figure 4.

Immunoblot analysis of the parental origin of PIR-A and PIR-B molecules produced by F₁ hybrids. Lysates of splenocytes from C57BL/6, BALB/c, [C57BL/6 (male) × BALB/c (female)] F₁, and [BALB/c (male) × C57BL/6 (female)] F₁ mice were immunoprecipitated with either the 6C1 or 10.4 anti-PIR antibodies. The immunoprecipitates were resolved by SDS/PAGE and were blotted onto a nitrocellulose membrane, and the PIR-A and PIR-B molecules were revealed by blotting with polyclonal anti-PIR antibodies.