Table 1.

Primers and conditions used for RT-PCR

Gene	Primers	Amplified product, bp	Cycles	Ta, °C
ACTIN	S: 5′-atctggcaccacaccttctacaatgagctgcg-3′; AS: 5′-cgtcatactcctgcttgctgatccacatctgc-3′	838	40	63
OCT4	S: 5′-gggaaggtattcagccaaacg-3′; AS: 5′-ggttcgctttctctttcggg-3′	180	20	56
BRACHYURY	S: 5′-cccgcgcactacacacccctcacc-3′; AS: 5′-ccttgggctgcggcgtcgtactg-3′	125	40	63
SCL	S: 5′-atggtgcagctgagtcctcc-3′; AS: 5′-tctcattcttgctgagcttc-3′	331	40	63
RUNX1B	S: 5′-cgtgcacatacattagtagcactacctttg-3′; AS: 5′-cttccacgaatcttgcttgcagaggttaag-3′	304	40	63
RUNX1C	S: 5′-gaagtctgaacccagcatagtggtcagcag-3′; AS: 5′-gtggacgtctctagaaggattcattccaag-3′	231	40	63
GATA-2	S: 5′-agccggcacctgttgtgcaa-3′; AS: 5′-tgacttctcctgcatgcact-3′	244	30	58
LMO2	S: 5′-ggatcctgccggagagactatctc-3′; AS: 5′-gaattcagtgaacacctccgcaaa-3′	289	40	63
C-MYB	S: 5′-ctcagacttggaaatgccttc-3′; AS: 5′-ccagtggtgtgagcagaaga-3′	406	40	63
VE-CADHERIN	S: 5′-accggatgaccaagtacagc-3′; AS: 5′-acacactttgggctggtagg-3′	596	40	63
FRIZZLED-3	S: 5′-ccttggcctgaagatatgg-3′; AS: 5′-acaaggaggtgaacaactacg-3′	210	40	56
FRIZZLED-4	S: 5′-ccaacatggctgttgaaatg-3′; AS: 5′-tcacccaaccatttcctctc-3′	168	40	56
FRIZZLED-5	S: 5′-tgctaccagccgtccttcagt-3′; AS: 5′-ccatgccgaagaagtagaccag-3′	319	40	56
WNT3A	S: 5′-ttcttactcctctgcagcctg-3′; AS: 5′-ctggatgccaatcttgatgc-3′	207	40	56
WNT5	S: 5′-cttcgcccaggttgtaattgaagc-3′; AS: 5′-ctgccaaaaacagaggtgttatcc-3′	273	40	56
WNT10B	S: 5′-aatgcgaatccacaacaac-3′; AS: 5′-gggtctcgctcacagaagtc-3′	288	40	56
GAPDH	S: 5′-gtcttctccaccatggagaaggct-3′; AS: 5′-catgccagtgagcttcccgttca-3′	277	40	50-63
CYCLIN-D1	S: 5′-gcggaggagaacaaacagat-3′; AS: 5′-tgtgaggcggtaggaca-3′	180	40	56

S indicates forward; and AS, reverse.