Table 1.

Oligonucleotides used for cloning and site-directed mutagenesis to generate retroviral expression vectors

Name	Sequence
mRsk1Kozak-T	ATA TAT CTC GAG GCC GCC ACC ATG CCG CTC GCC CAG CTC AAG
mRsk1-B	ATA TAT CTC GAG TCA CAG GGT GGT GGA TGG CAG CTT CC
mRsk1 K94A-T	GGG CAC TTG TAT GCC ATG GCA GTA TTA AAG AAG GCC ACG
mRsk1 K94A-B	CGT GGC CTT CTT TAA TAC TGC CAT GGC ATA CAA GTG CCC
mRsk1 K447A-T	ATG GAG TAT GCT GTC GCG GTC ATC GAC AAG AGC
mRsk2 Kozak:	GGA ATT AGA TCT CTC GAG GCC GCC ACC ATG CCG CTG GCG CAG CTG
mRsk2 K100A-T:	AGA CAG CTT TAT GCC ATG GCA GTA TTA AAG AAG GCC ACG CTG
mRsk2 K100A-B:	CAG CGT GGC CTT CTT TAA TAC TGC CAT GGC ATA AAG CTG TCT
mRsk2 K451A-T:	AAC ATG GAG TTT GCC GTG GCG ATT ATT GAT AAA AGC AAG AG
mRsk2 K451A-B:	CTC TTG CTT TTA TCA ATA ATC GCC ACG GCA AAC TCC ATG TT
mRsk1 K447A-B	GCT CTT GTC GAT GAC CGC GAC AGC ATA CTC CAT
mRsk3 Kozak-T	ATA TAT GTC GAC GCC GCC ACC ATG GAG CTG AGC ATG AAG AAG TTC
mRsk3-B	ATA TAT GTC GAC CTA CAA CCT GGT AGA CGT GAG TCT C
mRsk3	K91A-T GGT CAG CTC TAC GCC ATG GCG GTC CTG AAG AAA GCC ACC
mRsk3	K91A-B GGT GGC TTT CTT CAG GAC CGC CAT GGC GTA GAG CTG ACC
mRsk3	K444A-T GAT GCT GAG TAT GCT GTG GCG ATC ATC GAT AAG AGC AAA
mRsk3	K444A-B TTT GCT CTT ATC GAT GAT CGC CAC AGC ATA CTC AGC GTC
mRsk1 121	CAC CCG TTC GTG GTG AAG
mRsk1 271	CCC CAG TTT CTG AGC ACG
mRsk1 421	TGT AAG CGT TGT GTC CAC
mRsk1 571	GCA CCT GAG GTC CTG AAG
mRsk2 121	ATC TTG GTA GAA GTC AAT C
mRsk2 271	AAA GAT CGT AAA GAA ACA
mRsk2 421	GGA TAT GAA GTA AAA GAG
mRsk2 571	GAA AAT GGT CTT CTC ATG
mRsk3 121	ATT GTC AAG CTG CAT TAT G
mRsk3 271	ATC CTC AAA GCC AAG CTG
mRsk3 421	ATC GGG GTG GGC TCC TAC
mRsk3 571	CCC TGC TAT ACT GCA AAC
pRV 5′ Seq:	TTA TCC AGC CCT CAC TCC TTC

Underlined nucleotides are mutations. Sequencing primers are also listed.