Figure 2.

PBMC from a progressive MS patient were stained with mAb and isotype-matched control Ig, as described in the text. Two-color flow cytometry analysis of 1 × 10⁴ cells from each sample was performed. The percentage of double-positive cells from the lymphocyte populations analyzed is shown. A shows staining with an isotype control (mouse IgG1-FITC and mouse IgG1-PE) that is the control for B (CD4⁺ CCR5⁺ cells), C (CD8⁺ CCR5⁺ cells), D (CD4⁺ CXCR3⁺ cells), and E (CD8⁺ CXCR3⁺ cells).