Figure 4.

Bcl-X_L expression and DP apoptosis in PS KO × Bcl-X_L^flox/+ animals. Thymocytes from PS KO, PS KO × Bcl-X_L^flox/+, CD4-Cre, or control animals were harvested, protein were extracts collected, and Bcl-X_L level was measured by WB (A). Some animals from these same groups were treated intraperitoneally with anti-CD3, thymocytes were harvested 40 hours later, and the percentage of DP or SP thymocytes was measured by FACS (B). Each graphic is representative of 1 animal of 3 evaluated. (B) Value in each quadrant represents the percentage of each cell population inside the thymus.