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. 2007 Aug 10;110(9):3360–3364. doi: 10.1182/blood-2007-05-089326

Figure 1.

Figure 1

Lnk inhibits cytokine-independent and Tpo-stimulated growth in MPLW515L-expressing cells. (A-B) Real-time PCR (A) and Western blot (B) analysis of Lnk expression in Ba/F3 and UT7 cells stably expressing either MPLWT (WT) or MPLW515L (W515L). Relative mRNA expression levels are expressed in arbitrary units as a ratio of Lnk transcripts/18S transcripts (each value represents the mean (± SD) of 3 measurements of the sample). (C-D) BaF/MPLWT and BaF/MPL515 cells (C) were transfected by electroporation with either empty vector (EV), wild-type Lnk (LNKW), or an SH2 mutant Lnk (LNKM); UT7/MPL515 cells (D) were similarly transfected with either empty vector (EV), siRNA control vector (siC), or siLNK vectors (siL1 and siL2). Two days later, cells were cultured in selection media either with or without Tpo (1 ng/mL). Proliferation was measured by cell counts. Data represent the mean (± SD) of duplicate samples and are representative of 3 independent experiments. (E) Western blot analysis of Lnk expression in transfected Ba/F3 and UT7 cells. β-actin was used to control for equal loading and siRNA specificity.