Table 2.

PCR and sequencing primers used in pyrosequence analysis for the DNA-methylation assay

	Sequence	CpG dinucleotides
γ-globin
Set 1
PCR primer1	TTGATAAGGTAAATTTGATTAATAGTTTTA	na
PCR primer2	CCTCCTCTATAAAATAACCCATAAC	na
Set 2
PCR primer1	GAGTATTTAGTGAGGTTAGGGG	na
PCR primer2	Biotin-ATACTTCCTTTTATTCTTCATCCC	na
Sequencing primer	ATTTAGTGAGGTTAGGGG	−54 and −51
Set 3
PCR primer1	Biotin-TTGGTTAGGGATGAAGAATAAAAG	na
PCR primer2	CCTCCTCTATAAAATAACCCATAA	na
Sequencing primer1	AAAACTTATTAATAACCTCA	+5 and +16
Sequencing primer2	TCCTCTATAAAATAACCCAT	+49
β-globin
Set 4
PCR primer1	GTGTAATAAGAAAATTGGGAAAA	na
PCR primer2	Biotin-ATATCTCTTAACCCCATACCATCA	na
Sequencing primer1	TGTAATAAGAAAATTGGGAA	−306
Sequencing primer2	AGTTGTGATTTTAAATATTA	−265
Set 5
Forward	GGGTTGAGGGTTTGAAGTTTAATT	na
Reverse	Biotin-CCAACCCTAAAATATAACTCCACA	na
Sequencing primer	AGAAGAGTTAAGGATAGGTA	−125

na indicates not applicable.