Figure 2.

Reduced lymphocyte proliferative response to soluble contact antigen and normal proliferative response to Con A are seen in stromelysin-1-deficient mice. Lymphocytes were isolated from the cervical, axillary, and inguinal lymph nodes of animals sensitized and subjected to ear challenge with DNFB. (A) In vitro proliferative response to soluble form of the contact antigen, DNBS, was measured as described in Materials and Methods. (B) In vitro proliferative response to the mitogen Con A was measured as described in Materials and Methods. ♦, Wild-type mice; ▵, stromelysin-1-deficient mice; ○, naive mice. Each graph represents results from an experiment with n = 3; values are mean ± SEM. Each experiment was repeated three times with similar results.