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. 1999 Jun 8;96(12):6890–6895. doi: 10.1073/pnas.96.12.6890

Figure 5.

Figure 5

Enhanced BCR-mediated activation of Akt/PKB in primary B cells from Lyn-deficient mice. (A) Splenic B cells were isolated from wild-type C57BL/6 mice as described (26). B cells (2 × 107) were treated with okadaic acid for 2 hours before stimulation with 10 μg/ml F(ab′)2 goat anti-mouse Ig for 30 minutes. Equal amounts of cell lysates were immunoprecipitated with anti-Akt Ab and immunoblotted with anti-Akt (Lower) or anti-phosphoSer473-Akt (Upper). (B) Splenic B cells were isolated from both wild type and Lyn-deficient mice. Cells are stimulated with 10 μg/ml F(ab′)2 goat-anti-mouse Ig for 30 minutes. The phosphorylation state of Akt/PKB was measured as described in A.