Figure 3.
Effect of mAbs GK1.5 and IM7.8.1 on leukocyte subsets in vivo. Purified mAbs (250 μg) GK1.5, R1-2, IM7.8.1, or PBS were administered i.p. into naive (PL × SJL)F1 mice (n = 5 per treatment group). Peripheral blood leukocytes were isolated 24 h later for counting and fluorescence-activated cell sorter analysis with directly conjugated reagents for the percentage of B cells (B220+), T cells (T200+), and CD4+ T cells (mAb GK1.5+). Results are expressed as mean ± SD of absolute cell counts (×1,000/μl of blood) adjusted to the total white blood cell counts as determined by the Coulter Counter.