Table 2.
Ratios of red vs. green T cells in different organs of (PL × SJL)F1 mice 18-24 h after adoptive transfer
| Red:green cells coated with mAbs* | Cell mix at time of injection | Brain | Spleen | Liver |
|---|---|---|---|---|
| Antiintegrin α4 mAb vs. control mAb | ||||
| R1–2 (α4):53.7.3 (control), n = 2 | 1.0 | 0.78 | 0.89 | 0.97 |
| R1–2 (α4):GK 1.5(control), n = 2 | 1.0 | 0.79 | 0.96 | 1.2 |
| R1–2 (α4):53.2.1 (control), n = 2 | 1.0 | 0.89 | 1.2 | 1.1 |
| PS/2 (α4):53.7.3 (control), n = 2 | 1.0 | 0.50 | 0.82 | 1.0 |
| Anti-CD44 mAb vs. control mAb | ||||
| IM7.8.1 (CD44):53.7.3 (control), n = 2 | 1.0 | 0.49 | 1.0 | 1.3 |
| IM7.8.1 (CD44):53.2.1 (control), n = 2 | 1.0 | 0.39 | 1.0 | Not determined |
| Anti-CD44 mAb vs. antiintegrin α4 mAb | ||||
| R1–2 (α4):IM7.8.1 (CD44), n = 2 | 1.0 | 1.3 | 1.1 | 0.90 |
| Control | ||||
| No Ab:No Ab | 1.0 | 1.0 | 1.1 | 1.1 |
Each mouse received simultaneously two sets of 107 fluorescently labeled, antibody-treated T cells. The exact ratio of green and red cells in the initial cell mix was determined by fluorescence microscopy. Brain, spleen, and liver were harvested 5–24 h later and were snap-frozen. The results are given relative to the ratio of red vs. green T cells in various organs of sacrificed mice.