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Effect of exogenous TNF-α on PAI-1 expression in lean wild-type and TNFR-deficient lean mice. Lean mice or TNFR-deficient lean mice (four each, 20–24 weeks old) were injected i.p. with 4 μg of murine recombinant TNF-α (open bars) or saline (filled bars). Three hours later, the plasma (A) or adipose tissue (B) were collected and analyzed for PAI-1 antigen (A) or mRNA (B) as described in Materials and Methods. Error bars represent mean ± SD.
