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Effect of exogenous TNF-α treatment on TGF-β mRNA expression in lean wild-type and lean TNFR-deficient mice. Lean wild-type mice or TNFR-deficient lean mice (20–24 weeks old) were injected i.p. with 4 μg of murine recombinant TNF-α (open bars) or saline (filled bars). Three hours later, adipose tissues were collected and analyzed for TGF-β mRNA levels as described in Materials and Methods. n = 3, error bars represent ± SD.
