Figure 5.

Growth inhibition of LIM1215 human colon carcinoma cells by prodoxorubicin in the presence of antibody 38C2. (A) Growth inhibition assay. Cells in quadruplicate wells in a 96-well plate were lysed 120 hr after drug addition, and the activity of the cytoplasmic enzyme lactate dehydrogenase released from the cells was detected by using a colorimetric assay. The intensity of the red color correlates with the number of cells in the well. (B) Growth inhibition in the presence of two fixed concentrations of prodoxorubicin and increasing concentrations of antibody 38C2. Controls are shown in black columns. dox, doxorubicin; prodox, prodoxorubicin; MVK, methyl vinyl ketone. Bars indicate SD; n = 4.