Figure 2.
Analysis of the butanolic extract of nat-LDL (a) and mox-LDL (b). Extracts were separated by TLC (15, 16), visualized by exposure to iodine vapor (Bottom), and scanned for radioactivity of [3H]LPA added as tracer to nat-LDL and mox-LDL before extraction (Middle). Areas of silica gel of equal length from the origin to the top of the TLC plate were scraped off, eluted, and assayed for shape change (Top). Shape change tracings of LPA eluates from nat-LDL and mox-LDL are shown to the right of a and b. The experiment is representative of three others. (c) Biologically active LPA increases in mox-LDL and mm-LDL. LPA from nat-LDL, mox-LDL, and mm-LDL was extracted and separated by TLC, and shape change was quantified against a standard curve of 1-palmitoyl LPA. Values are mean ± SD from 11 individual LDL preparations (represented by different symbols) before (nat-LDL) and after (mox-LDL) mild oxidation and from 8 preparations of LDL after spontaneous oxidation (mm-LDL).