Figure 3.

mox-LDL induces cell rounding and actin stress-fiber formation in confluent human endothelial cells through activation of the LPA receptor. Cells were incubated for 2 min at 37°C with 0.1% ethanol (a; control), 250 μg/ml mox-LDL (b), 1 μM LPA (c), 250 μg/ml nat-LDL (d), LPA eluate from 250 μg of nat-LDL (e), or LPA eluate from 250 μg of mox-LDL (f). (g–i) Cells were also pretreated with NPTyrPA (10 μM) for 60 min before incubation with mox-LDL (g), LPA (h), or thrombin (i; 1 unit/ml). Cells were stained for F-actin with rhodamine phalloidine. The experiment is representative of four others that gave similar results. (Bar = 30 μm.)