Table 2.
Comparison of shape change induced by mox-LDL and by native LDL spiked with LPA extracted from mox-LDL
| Shape change, % | |
|---|---|
| LPA extract of mox-LDL | 100 |
| mox-LDL | 32 ± 14 |
| LPA extract of mox-LDL + nat-LDL | 27 ± 10 |
| nat-LDL | 4 ± 2 |
| Blank extract + nat-LDL | 5 ± 2 |
The biological activity of LPA extracted from mox-LDL was compared with the biological activity of the respective mox-LDL. The amount of LPA extracts added to the platelet suspension ranged from 3 pmol to 12 pmol and was equivalent to the amount of mox-LDL from which LPA was extracted. For spiking of nat-LDL with the LPA extract of mox-LDL, ethanol of the LPA extract was evaporated and incubated for 10–15 min at 37°C with the respective native LDL from which mox-LDL has been prepared. Incorporation of LPA into nat-LDL was >90% as measured by radioactivity of [3H]LPA. The same amount of nat-LDL and mox-LDL (5–15 μg of LDL protein) was added to the platelet suspension. The activity of the LPA extract of mox-LDL in individual experiments was set to 100%. Values are mean ± SD of nine experiments with different LDL preparations. Blank extract is eluate from blank silica gel.