Table 1.
P19ARF blocks nucleo-cytoplasmic shuttling of Mdm2
| Transfected genes | Total number of heterokaryons examined | Number of heterokaryons in which Mdm2 shuttles | Percentage of heterokaryons in which Mdm2 shuttles |
|---|---|---|---|
| Mdm2 | 32 | 27 | 84.4 |
| Mdm2 and p19ARF | 26 | 0 | 0 |
HeLa cells were transfected with an expression plasmid for Mdm2 or cotransfected with plasmids encoding Mdm2 and p19ARF. Thirty-six hours after transfection, the nucleo-cytoplasmic shuttling of Mdm2 was determined by the heterokaryon assay as described. The heterokaryons that contained at least one transfected HeLa nucleus and one 2KO nucleus were randomly chosen and examined for Mdm2 shuttling.