Table 3.

Sequence analysis of the p53 gene (exons 4–9) of the primary tumor and of the p53 cDNA of the respective micrometastatic cell lines

Patient identifier	Tumor type	Tumor stage	Primary tumor p53 gene		Micrometaststic cell lines p53 cDNA
			Sequenced exons	Mutation, codon	Sequenced region, bp	Mutation, codon
PC-E1	Prostate Ca	pT3N0M0	4,5,6,7,8,9	No*	215–1396	No*
PC-R1	Prostate Ca	pT4N0M0	4,5,6,7,8,9	No*	179–1369	No*
PC-S1	Prostate Ca	pT3N0M0	n.d.	n.d.	178–1396	No*
PC-L1	Prostate Ca	pT3N0M0	n.d.	n.d.	174–1396	No*
PC-H1	Prostate Ca	pT3N0M0	4,5,6,7,8,9	273, CGT → TGT*	215–1396	No*
				284, ACA → ATA
LC-D1	Lung Ca	pT3N2M0	4,5,6,7,8,9	No**	215–1396	No*
LC-M1	Lung Ca	pT4N0M0	4,5,6,7,8,9	No*	173–1396	No*
CC-B1	Colon Ca	pT3N0M0	n.d.	n.d.	215–1396	No
BC-H1	Breast Ca	pT2N0M0	4,5,6,7,8,9	No*	215–1396	No*
BC-K1	Breast Ca	pT1N0M0	4,5,6,7,8,9	169, Δ15 bp*	215–1396	No*

The p53 cDNA has a length of 1,760 bp. The start codon ATG begins at bp 215 and the stop codon ends at bp 1386 of the p53 cDNA. n.d., not determined. 

^*Wild-type p53 sequences with the homozygous BstUI polymorphism CGC at codon 72; the other p53 sequences have CCC at codon 72. 

^**Heterozygous for the BstUI polymorphism.