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. 1999 Jun 8;96(12):6947–6952. doi: 10.1073/pnas.96.12.6947

Figure 4.

Figure 4

Mapping the regions of REA required for repression of ER activity. The regions of REA required for repression of ER activity were monitored by using the N- and C-terminal truncated REAs indicated. Repressive activity was monitored by the ability of the cotransfected REA to repress ER-stimulated transcription as measured by CAT assay from the estrogen-responsive reporter (ERE)2-pS2-CAT. MDA-MB-231 human breast cancer cells were cotransfected with 100 ng pCMV-ERα, 500 ng of pCMV-REA construct, and internal control β-galactosidase plasmid. Cells were treated with 10−8 M E2 for 24 h. The level of repression of ER activity by full-length REA is set at 100%. The effectiveness of the truncated forms of REA in repressing ER activity is listed as a percentage of full-length REA. Values are the means ± SD of three to eight determinations. REA (1–226) was found to suppress ER activity to the same level as full-length REA when higher amounts of REA (1–226) expression plasmid were utilized.