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Binding activity of human scFvs on SW1990 cells measured by using FACS. Cells were incubated in PBS for 1 h at 4°C with (A) 1% BSA; negative control; (B) S6; (C) S7; (D) S8; and (E) S10. Binding of soluble fragments was detected by adding first anti-Flag M2 murine mAb followed by FITC-conjugated antimurine F(ab′)2.
