. 1999 Jun 8;96(12):6953–6958. doi: 10.1073/pnas.96.12.6953

Table 2.

Complete protein sequences

Clone	Heavy chain	Light chain
`FR1`
`S6`	`QVQLVESGAEVKKPGASVKVSCKASGYTFT`	`QAVLTQPSSLSASPGASVSLTC`
`S7`	`QVQLQESGGGLVQPGGSLRLSCVASGFTFR`	`QAVLTQPSSLSASPGASASLTC`
`S8`	`HVQLVQSGAEVKKPGASVKVSCKASGYTFT`	`QAVLTQPSSLSASPGASVSLTC`
`S10`	`QVQLVQSGAEVKKPGASVKVSCKASGYTFT`	`QAVLTQPSSLSASPGASVSLIC`
`CDR1`
`S6`	`GYYMH`	`TLRSGINVGAYRIY`
`S7`	`SYAMS`	`TLRSDINVRSYRIY`
`S8`	`GYYMH`	`TLRSGINVGAYRIY`
`S10`	`GYYMH`	`TLRSGINVGAYRIY`
`FR2`
`S6`	`WVRQAPGQGLEWMG`	`WYQQKPGSPPQFLLR`
`S7`	`WVRQAPGKGLEWVS`	`WYQQKPGSPPQYLLR`
`S8`	`WVRQAPGQGLEWMG`	`WYQQKPGSPPQFLLR`
`S10`	`WVRQAPGQGLEWMG`	`WYQQKPGSPPQFLLR`
`CDR2`
`S6`	`WINPNSGGTNYAQKFQG`	`YKSDSDKQQGS`
`S7`	`TISGSGGSTYYADSVKG`	`YKSDSDKHQGS`
`S8`	`WINPNSGGTNYAQKFQG`	`YKSDSDKQQGS`
`S10`	`WINPNTGGTKYTQKFQG`	`YKSDSDKQQGS`
`FR3`
`S6`	`RVTMTRDTSISTAYMELSRLRSDDTAVYYCRQ`	`GVPSRFSGSRDASANAGILLISGLQSEDEADYYC`
`S7`	`RFTISRDNAKNSLYLQMNSLRAEDTAVYYCGR`	`GVPSRFSGSRDASANAGILVISGLQSEDEADYYC`
`S8`	`RVTMTRDTSISTAYMELSRLRSDDTAVYYCAR`	`GVPSRFSGSRDASANAGILLISGLRSEDEADYYC`
`S10`	`RVTMTRDTSISTAYMELSRLRSDDTAVYYCAR`	`GVPSRFSGSRDASANAGILLISGLRSEDEADYYC`
`CDR3`
`S6`	`GGYGPRGYFDI`	`AIWHSSAWV`
`S7`	`SVVGY`	`AIWHSSTWV`
`S8`	`AGRFGELY`	`AIWHSSAWV`
`S10`	`VKSGAFDI`	`AIWHSSAWV`
`FR4`
`S6`	`WGQRDHGHRLLS`	`FGGGTQLTVLG`
`S7`	`SQGTLVTVSS`	`FGGGTKLTVLG`
`S8`	`WGQGTLVTVSS`	`FGGGTQLTVLG`
`S10`	`WGQGTMVTVSS`	`FGGGTKLTVLG`

The boldface letters (from FR1 through FR3) indicate the amino acid differences between V_H and V_L domains of scFv clones and the closest germ-line V segments. FR, framework region; CDR, complementarity-determining region.