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Kinetics and thermodynamics of binding of sLex and Lex conjugates to scFvs
| scFv | sLex-biotin
|
Lex-BSA
|
||||
|---|---|---|---|---|---|---|
| kon (×104 M−1 s−1) | koff (×10−3 s−1) | Kd (×10−7 M) | kon (×104 M−1 s−1) | koff (×10−3 s−1) | Kd (×10−7 M) | |
| S6 | 4.3 | 4.7 | 1.1 | 1.3 | 8.3 | 6.2 |
| S7 | 1.1 | 5.3 | 4.9 | 2.6 | 7.3 | 2.9 |
| S8 | 5.1 | 9.1 | 1.8 | 2.3 | 6.5 | 2.9 |
| S10 | 1.2 | 6.7 | 5.7 | 2.1 | 4.6 | 2.2 |
The kinetic constants of scFvs were measured using the BIAcore biosensor: kon, association rate constant; koff, dissociation rate constant; Kd, calculated (Kd = koff/kon).
