Table 4.

A BIAcore two-site binding assay of scFvs and sLe^x

Second scFv	Immobilized scFv*
	S7	S8	S10
S6	+	+	+
S7	−	ND	ND
S8	+	−	+
S10	+	ND	−

^*Immobilized scFv was used to trap sLe^x. The second scFv was then tested for binding to the scFv–sLe^x complex. Plus signs (+) indicate scFvs that bound concurrently (typically 100–500 response units) and hence recognized different regions of sLe^x. Minus signs (−) indicate scFvs that did not bind concurrently (typically 0–20 response units). ND, not determined.