Figure 1.

Coinjection of CpG-DNA and Leishmania lysate stimulates IFN-γ production. Lymph node cell suspensions were prepared from BALB/c mice 9 days after their ears were injected with F/T killed parasite lysate alone (▴) or in combination with CpG-ODN 1826 (■), ODN 1911 (▵), CpG-ODN 1758 (░⃞), or CpG-ODN 1835 (□) and assayed for parasite-specific lymphocyte proliferation (A), IFN-γ release (B), or IL-4 production (C), as described in Materials and Methods. Results are presented as the mean (± SD) of assays performed in triplicate. The data are representative of three experiments.