Table 3.

Therapeutic effects of CpG-ODN depend on IL-12 production

Treatment group	Survival at 6 weeks
BALB/c injected with L. major parasites
1. CpG-ODN 1826 + anti-IL12 Ab	0/20 (0%)
2. CpG-ODN 1826 + rat IgG	17/20 (85%)
3. CpG-ODN 1826 + PBS	16/20 (80%)
4. PBS + PBS	4/20 (20%)
C57BL/6 + L. major parasites
1. Anti-IL-12 Ab	2/15 (13%)
2. Rat IgG	13/15 (87%)
3. PBS	9/10 (90%)

After i.p. injection of anti-IL-12 mAb, rat IgG, or PBS, the footpads of mice were infected with L. major parasites and then injected with ODN 1826 as described in Materials and Methods. Animals were euthanized if footpad thickness decreased mobility or if ulceration and necrosis developed.