Figure 2.
Focus formation by clones in 1° assays of cells from low-density passages. The six clones were passaged 56 times at low density and were used for a 1° assay of 105 cells at various passage levels. Selected assays from three of the clones are shown here to illustrate the diverse clone-specific dynamics of transformation during the low-density passages. The clones are listed at the top of each column. The series numbers precede the 1° assay designation on the left. The number of LDPs of each series were first series, 5; third series, 21; sixth series, 56. Note the tiny dense foci of clone 1A assays 1–1° and 3–1°, plus a larger dense focus in 3–1°. There are light foci in clone 6H assays 3–1° and 6–1°, and in 3E assay 3–1°. There are many light foci in clone 3E assay 6–1°, plus small dense foci. See Table 1.