Table 2.
Focus formation by clones in serial assays at confluence
| Clone | Focus formers/105 cells
|
||||
|---|---|---|---|---|---|
| 1° | 2° | 3° | 4° | 5° | |
| 1A | 4*–7 | 43–0 | 18,100–0 | 4,800–0 | 17,600–1.200 |
| 3F | 0–0 | 0–116 | 60–56 | 22–300 | 6,000–6,000 |
| 2B | 0–0 | 1–125 | 0–80 | 6–250 M | 200–4,000 |
| 6H | 0–0 | 0–TNTC | 15,000–0 | 4,000–0 | 10,000–0 |
| 4B | 0–0 | 0–1 | 0–1 | 0–200 | 1–TNTC |
| 3E | 0–0 | 11*–4 | 1,450*–0 | 8,500*–0 | 11,000–0 |
| A′† | 0–0 | 0–0 | 0–0 | 0–0 | ND |
The cells used in these serial assays of the clones were from the first series of such assays that were initiated after five low-density passages. The numbers of foci are all normalized to 105 cells, but where they exceed 250, they are extrapolated from dilutions of the cells mixed with a standard passage of nontransformed A′ to form a confluent background. The first number of each pair denotes dense foci (≥1 mm unless noted with ∗); the second number denotes light foci. See photograph in Fig. 3. ∗, ≪1 mm; M, mottled; TNTC, too numerous to count.
†
The serial assays for the uncloned A′ cells are taken from the fourth series.