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The presence of CRSP subunits correlates with the CRSP transcription activity. (a) Reconstituted transcription reactions with GC box template, Sp1, RNA polymerase II and basal factor fraction, and IP-TFIID (lanes 1–10) supplemented with various fractions derived from glycerol gradient centrifugation. (b) Western blots of the glycerol gradient fractions probed with anti-CRSP150 or anti-CRSP33 antibodies.
