Table 1.

Purification table for CRSP starting with 100 liters of HeLa cells

Chromatographic procedures	Total protein amount	Approximate fold purification
Nuclear extract	2 g
Ammonium sulfate ppt.	1 g	2
Phosphocellulose	40 mg	40
Ni-NTA-agarose	3 mg	1,000
Blue sepharose	1 mg	2,400
Poros heparin	0.5 mg	3,000
Glycerol gradient	0.05 mg	9,000
MonoS	.004 mg	45,000