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. 1999 Jun 8;96(12):6982–6987. doi: 10.1073/pnas.96.12.6982

Figure 5.

Figure 5

Complementation of S. cerevisiae mutant strains by human ALG5 and ALG6. (a) Western blot analysis of carboxypeptidase Y (mCPY) showing the glycoforms lacking one (−1) or two (−2) oligosaccharide chains. In the left panel, alg5 yeasts transformed with the vector (pYEp352), with pYEp352-huALG5 (huALG5), and with pYEp352-yeastALG5 (yeastALG5) are indicated. The right panel shows CPY glycoforms in alg6 yeasts transformed with pYEp352 alone, with pYEp352-huALG6, and with pYEp352-huALG6[A333V]. (b) Growth of alg6wbp1–2 yeasts transformed with pYEp352, pYEp352-huALG6, and pYEp352-huALG6[A333V] constructs. Tenfold dilutions starting from the left with the same cell number were plated on YPgalactose plates and were incubated at 25°C for 5 days.