Figure 3.

Dose-dependent induction of the AN2 and AN5 truncated forms of the human 1α-OHase promoter by PTH (1–34). AN2- (1,100 bp, filled bar) and AN5- (463 bp, open bar) truncated forms of the human 1α-OHase promoter were inserted into a luciferase reporter expression vector, transfected into AOK-B50 kidney cells, and treated with increasing concentrations of PTH (1–34) for 24 hr. The AOK-B50 cells were then assayed for luciferase activity. Each bar represents the mean fold induction in luciferase activity relative to that of the promoterless construct in the absence of PTH ± SEM of three independent determinations. ∗, P < 0.01 vs. the same construct in the absence of PTH.