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. Author manuscript; available in PMC: 2008 Mar 2.
Published in final edited form as: J Mol Biol. 2006 Dec 6;366(4):1142–1160. doi: 10.1016/j.jmb.2006.11.090

Figure 3.

Figure 3

Thermostability of Ad5 and pV-deletion mutants (a) Thermostability analysis of Ad5, Ad5-dV and Ad5-dV/TSB particles. Purified viral particles (1010 VP) were incubated at various temperatures for 3 days and the resulting infectivity was determined by titration in a triplicate plaque assay on A549 cells. The residual (as % of the infectious titer at −80ºC after 3 days) infectious titers following incubation of the viruses at various temperatures for 3 days were plotted. (b–d) The kinetics of virus thermo-inactivation. 1010 VP of Ad5 (b), Ad5-dV (c) and Ad5-dV/TSB (d) viruses were incubated at various temperatures for 1, 3 and 7 days and the resulting infectious titers were determined by titration and plotted as an average in triplicate plaque assay on A549 cells.