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. Author manuscript; available in PMC: 2008 Jan 16.
Published in final edited form as: Virus Res. 2006 Aug 22;122(1-2):144–153. doi: 10.1016/j.virusres.2006.07.009

Fig. 3.

Fig. 3

Recombinant Ad fiber proteins from Ad subspecies B1 and B2 specifically block CD80 and CD86-mediated gene transfer of Ad5/3 pseudotyped vectors. Cells were pre-incubated with indicated amounts of recombinant Ad7 or Ad11 fiber. The cells were then incubated with Ad vectors (100 vp/cell) or no virus, to determine the background luciferase activity. Virus was removed and the cells were grown in growth media. After 36 h the cells were lysed and the luciferase assay was performed. Recombinant Ad7 fiber and Ad11 fiber proteins specifically block Ad5/3 pseudotyped vector (Ad5/3luc1) luciferase gene transfer (A and C) but have no effect on Ad5 (Ad5luc1) gene transfer (B and D). Numbers on the horizontal axes are the recombinant fiber concentrations in μg/ml. Relative light units normalized by protein concentration in the sample (RLU/mg). Error bars = ±S.D.