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. Author manuscript; available in PMC: 2008 Jan 16.
Published in final edited form as: Virus Res. 2006 Aug 22;122(1-2):144–153. doi: 10.1016/j.virusres.2006.07.009

Fig. 4.

Fig. 4

Members of Ad species B exhibit specific binding to cells expressing CD80 or CD86. Binding of Ad serotypes with HeLa (black bars), CHO (white bars), CHO-CD80 (dark gray bars), and CHO-CD86 (light gray bars) cells was analyzed. Cells were harvested with Versene and incubated without virus or with 100 vp/cell of Ad3, Ad5, Ad7, Ad11, Ad14, Ad16, Ad21, Ad34, Ad35, or Ad50 for 1.5 h at 4 °C. Cells were washed and incubated with anti-Ad hexon rabbit antibodies followed by anti-rabbit IgG antibodies conjugated to Alexa 488 and analyzed by flow cytometry. The labeled cells were gated and scored as positive or negative and the percent of positive cells were plotted for each virus. All tested Ad species B members showed increased binding to CHO-CD80 and CHO-CD86 vs. CHO cells. Ad5 did not show any increased binding to CHO-CD80 or CHO-CD86 vs. CHO cells. Error bars = ±S.D.