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Equilibrium transitions of the NGF-coupled pro-peptide and the isolated pro-peptide. The proteins were incubated in 50 mM Na-phosphate, pH 7.0 at various GdmCl concentrations for 16 h at 20°C. Fluorescence signals of the NGF-coupled pro-peptide (•, 20 μg/mL, excitation: 280 nm, emission: 325 nm) and the isolated pro-peptide (○, 50 μg/mL, excitation: 250 nm, emission: 345 nm) were recorded.
