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. 2007 Apr;16(4):644–653. doi: 10.1110/ps.062478607

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Figure 6. — Chaperone-like activity of HIV-1 protease. (A) Aggregation assay with chemically denatured rhodanese: Rhodanese in the absence of HIV-1 protease (▴); a 2.5-fold (▪) or fivefold (♦) molar excess of HPR-M, a 2.5-fold () or fivefold () molar excess of HPR-DS; a fivefold molar excess of BSA (▵). (B) Effect of HIV-1 protease inhibitor: Rhodanese in the absence (▴) or presence of a fivefold molar (•) excess of HPR-WT; a fivefold molar excess of HPR-WT inhibited with HIV-1 protease inhibitor (○). (B) (Bottom) SDS-PAGE analysis of the assay solutions to exclude remnant proteolytic activity of HPR-WT.

Inline graphic — Chaperone-like activity of HIV-1 protease. (A) Aggregation assay with chemically denatured rhodanese: Rhodanese in the absence of HIV-1 protease (▴); a 2.5-fold (▪) or fivefold (♦) molar excess of HPR-M, a 2.5-fold () or fivefold () molar excess of HPR-DS; a fivefold molar excess of BSA (▵). (B) Effect of HIV-1 protease inhibitor: Rhodanese in the absence (▴) or presence of a fivefold molar (•) excess of HPR-WT; a fivefold molar excess of HPR-WT inhibited with HIV-1 protease inhibitor (○). (B) (Bottom) SDS-PAGE analysis of the assay solutions to exclude remnant proteolytic activity of HPR-WT.