Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
SDS-PAGE (12% Tris-glycine) of the purified Leu67Tyr/Ala205Leu cathepsin L mutant. (Lane 1) Crude supernatant fraction of the human Leu67Tyr/Ala205Leu mutant from P. pastoris; (lane 2) activation of the precursor mutant by treatment with pepsin; (lane 3) purified Leu67Tyr/Ala205Leu mutant after passage through the Butyl-Sepharose column; (lane 4) purified recombinant wild-type human cathepsin L. Molecular mass standards are reported in the left lane (Coomassie-blue staining).
