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. 2007 Aug;16(8):1751–1761. doi: 10.1110/ps.072846407

Figure 1.

Figure 1.

Cleavage experiment of the fusion proteins MBP-H10-AVI and MBP-H0-AVI with CoCl2. Control protein MBP-H0-AVI in buffer (lane 1), with ascorbate and hydrogen peroxide (lane 2), and with ascorbate hydrogen peroxide and CoCl2 (lane 3). MBP-H10-AVI protein in only buffer with ascorbate and hydrogen peroxide (lane 4) and with CoCl2, ascorbate, and hydrogen peroxide (lane 5). In lane 6, the protein was treated as in lane 5 but with EDTA added prior to the CoCl2. In lane 7 the fusion protein was incubated with CoCl2 but without ascorbate or hydrogen peroxide.