Figure 2.

Two-dimensional ¹H–¹⁵N HSQC spectrum of uniformly ¹³C/¹⁵N-labeled zf-HIT domain of ZNHIT2 (600 MHz, 298K, protein concentration 1.1 mM in 20 mM phosphate buffer at pH 6.0, 100 mM NaCl). Amino acid assignments of each peak, according to the correlation of the ¹H–¹⁵N atoms of an amino acid, are shown. Horizontal lines identify side-chain NH₂ groups of asparagine and glutamine residues.