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DNA-binding assay with the zf-HIT domain. Binding of the indicated protein to the cytomegalovirus promoter (CMV-TATA) DNA was monitored by a Biacore 3000 instrument. Each injection started at 60 and ended at 120 sec. The binding activity of the proteins and the CMV-TATA DNA was indicated as the difference in the resonance units (RU) between the DNA-bound and -unbound flow cells.
