Table 2.

VP39-F180W binding affinities for m⁷G, m⁷G(5′)pp, and methylated nucleobases

Protein mutant	Ligand	Kd, μM*
F180W	m7G	7.6
F180W	m7G(5′)pp	14.6
F180W	m7G(5′)pp†	19.1
F180W	m3Ade	86.0
F180W	m1Ade	97.9
F180W	m3Cyt	85.5
F180W	m1Cyt	144.0

^*Determined by fluorescence titration at pH 5.8 (see Materials and Methods). 

^†Reduced and reoxidized.