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Southern blot hybridization of GTP-labeled Twort RNA to genomic DNA. Twort DNA was digested with PstI (P), XbaI (X), KpnI (K), NsiI (N), EcoRI (E), SalI (Sl), SacI (Sc), and DraI (D) and transferred. The blot was probed with 50 μg 15-min Twort RNA that was end-labeled with [α-32P] GTP under self-splicing conditions. Sizes of BstEII digested λ DNA fragments are indicated to the right.
