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. Author manuscript; available in PMC: 2008 Jan 16.
Published in final edited form as: Gastroenterology. 2006 Nov 7;132(1):384–396. doi: 10.1053/j.gastro.2006.10.037

Figure 3. CpG island methylation within the socs-3 promoter is observed in Mz-ChA-1 but not H69 cells.

Figure 3

Genomic DNA extracted from the cell lines was treated with bisulfite and then subjected to methylation-specific PCR (A) using the methylated DNA- (M) and unmethylated DNA- (U) specific primer sets. PCR products were sequenced for the 44 CpG sites located between nucleotides -678 and -216 of the socs-3 promoter as described in the Materials and Methods section (B). The horizontal squares represent CpG islands while the vertical squares represent the individual 5 clones sequenced. Each black square represents a methylated cytosine residue within the CpG islands.