Table 1.

Sequences for primers employed in this study

	Primer pair	Annealing temperature (cycle)	Product size
socs-3 gene sequence
5′-UTR to exon 1	F: 5′-TGCAGAGTAGTGACTAAACATTACAAGAA-3′	58°C (35)	235
	R: 5′-GGAGGGGACCAGGAGAGG-3′
exon 1 to intron	F: 5′-CGACTTGGACTCCCTGCT-3′	56°C (35)	392
	R: 5′-GAGGGGAAACCGGGAAAAG-3′
intron to exon 2	F: 5′-CTCAGTCCCGGGAGCTTTTC-3′	58°C (35)	715
	R: 5′-GCTGGTACTCGCTGTTGGAG-3′
exon 2	F: 5′-GGTCACCCACAGCAAGTTT-3′	55°C (35)	676
	R: 5′-TTAAAGCGGGGCATCGTACT-3′
exon 2 to 3′-UTR	F: 5′-AGTACGATGCCCCGCTTT-3′	55°C (35)	670
	R: 5′-GCACCAGGTAGACTTTGGAAC-3′
3′-UTR	F: 5′-CCTGGTGACATGCTCCTCT-3′	55°C (35)	629
	R: 5′-CAGGTAATTCCATCGCTGCT-3′
3′-UTR	F: 5′-TCATTGGAGAGGCTGGACTG-3′	55°C (35)	400
	R: 5′-GCAAAGTTTGACTTGGATTGG-3′
Methylation specific PCR
Methylated	F: 5′-GGAGATTTTAGGTTTTCGGAATATTTC-3′	58°C (35)	142
	R: 5′-CCCCCGAAACTACCTAAACGCCG-3′
Unmethylated	F: 5′-GTTGGAGTTTTAGGTTTTTGGTATTTT-3′	60°C (32)	147
	R: 5′-AAACCCCAAAACTACCTAAACACCA-3′
Bisulfite sequencing	F: 5′-GATTTGAGGGGGTTTAGTTTTAAGGA-3′	58°C (35)	519
	R: 5′-CCACTACCCCAAAAACCCTCTCCTAA-3′
Real-time PCR for socs-3	F: 5′-TTCTACTGGAGCGCAGTGAC-3′	60°C (40)	127
	R: 5′-ACTGGGTCTTGACGCTGAG-3′

UTR, untranslated region; F, forward; R, reverse; socs-3, suppressor of cytokine signaling 3