Figure 1.

Allele-specific DNase1 sensitivity assay. (A) Left, autoradiograph illustrating SNuPE reactions detecting polymorphism between Bl6 and M. spretus (Spr) U2af-rs1 alleles (G and A, respectively). Right, autoradiographs illustrating relative levels of alleles in (C57BL6 × M. spretus) F₁ DNA prepared from nuclei treated with various concentrations of DNase1. SNuPE reactions were loaded in duplicate. (B) Quantitation of data shown above (Expt1) and an independent experiment (Expt2) showing expressed allele as % of total signal. The result expected if both alleles exhibit equal DNase1 sensitivity (Null) is included for illustrative purposes. (C) Quantitation of two independent allele-specific DNase1 sensitivity assays for the Pgk-1 locus. Data is shown for (PGK × T16H) F₁ female (T16H), and for control (PGK × 129) F₁ female (F₁). Data points in B and C are mean values of duplicate loadings.