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Histone acetylation assay. (A) Hybridization of heterochromatin and genic probes to slot blots with triplicate loading of ≈100 ng of input DNA (I) or DNA from antibody-bound (BD) and -unbound (UB) chromatin fractions without (−Ab) or with (+Ab) antibody to hyperacetylated histone H4 isoforms. (B) Ratio of signal for H4-bound/H4-unbound fractions calculated from three independent determinations. Slot blot data was quantified by using PhosphorImager analysis.
